| 質粒名稱: | ANTI-CD11c IMMUNOTOXIN |
|---|---|
| 出品公司: | Addgene |
| 目錄編號: | 22850 |
| 存儲實驗室: | Jose Conejo-Garcia |
|
載體骨架基本信息 |
|
| 載體名稱: | pET-28 a(+) |
| 載體出品公司: | Clontech |
| 空載體大小: | 5369bp |
| 完整質粒大小: | 5369bp |
| 載體類型: | 細菌表達 |
| 載體抗性: | Kanamycin, 50 μg/mL |
| 生長溫度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷貝數: | 未知 |
|
插入基因信息 |
|
| 插入基因名稱: | ANTI-CD11C IMMUNOTOXIN |
| 物種來源: | hamster, mouse, psedomonas |
| 插入大小: | 2035bp |
| 克隆方法: | 限制性內切酶 |
| 5’克隆位點: | NcoI (not destroyed) |
| 3’克隆位點: | EcoRI (not destroyed) |
| 5’測序引物 | T7 promoter |
| 3’測序引物 | T7 terminator |
|
文獻引用方法 |
|
| 材料和方法部分: | ANTI-CD11c IMMUNOTOXIN was a gift from Jose Conejo-Garcia (Addgene plasmid # 22850) |
| 參考文獻部分: | Depletion of dendritic cells delays ovarian cancer progression by boosting antitumor immunity. Huarte E, Cubillos-Ruiz JR, Nesbeth YC, Scarlett UK, Martinez DG, Buckanovich RJ, Benencia F, Stan RV, Keler T, Sarobe P, Sentman CL, Conejo-Garcia JR. Cancer Res. 2008 Sep 15. 68(18):7684-91. 10.1158/0008-5472.CAN-08-1167 PubMed 18768667 |
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